Day 2 :
- Applications of Biotechnology | Cell Culture | Microbial Biotechnology
Location: Esperanto I+II
Kristian M Muller
Bielefeld University, Germany
St. Mary’s Junior College of Medicine, Nursing and Management, Taiwan
Li Yu-Jung is currently a Lecturer at St. Mary’s Medicine, Nursing and Management College. He has completed his training program of Oral and Maxillofacial Surgery in Veterans General Hospital, Taipei, Taiwan during 2002-2006. He is also a Doctoral candidate majored in Mechanical and Electrical Engineering from National Taipei University of Technology. He has received his MS degree of Clinical Dental Science from Institute of Clinical Dentistry, National Yang-Ming University. He has also received MS degrees of Chemistry and Biophysics from Graduate Institute of Biophysics, National Central University and Institute of Chemistry, Tamkang University during 2006-2010 and Bachelor’s degree of Dentistry from Department of Dentistry, Chung Shan Medical University in 2002.
Traditionally the tooth sensations are mainly from its inside pulp structure and outside periodontal ligament (PDL). However, both of them will be taken off while dental implant replacement and the fact may allow us to design the drug delivery and bio-sensing modules above it to reach the inside bone marrow and the surrounding blood pool. Such device may provide the application for long-term, painless and continuous drug delivery and bio-sensing. The drug delivery module is composed by the piezoelectric micro-pump, the drug container and the power supply battery. While the bio-sensor has integrated circuit (IC), Bluetooth 4.0 module and the power supply inside. The device may improve the life quality toward those patients with chronic and critical diseases suffering from frequently invasive procedures. For example, patients with diabetes mellitus (DM) may need four invasive blood sugar detection and subcutaneous injections of insulin to control blood sugar levels each day in the later stage traditionally. With such device, they may avoid such frequently invasive procedures. Currently the in vitro experiments, simulations and the preliminary canine study provide the positive results and such pathway is proven efficiency in medical and dental practice. However, the development of this device is just beginning and further improvements are needed to overcome the technical challenges including safety concern, module size minimizing and power supply prolong. Standard protocol establishment in dental and medical practice is also important. With proper improvement and technology support, the intra-oral device may provide further medical applications.
Harbin Institute of Technology, China
A recyclable catalyst of sliver nanoparticles well dispersed in mesoporous silica was successfully synthesized via a straight-forward strategy combining electrospinning technique with post-calcination. The resulted mats containing silver nanoparticles of 10±4 nm size well dispersed in mesoporous silica fibers of 380±80 nm diameter presented surface plasma resonance of silver nanoparticles at 420 nm. The catalytic behavior of the nanofiber on the reduction of methylene blue by NaBH4 was tracked and showed that the silver nanoparticles immobilized on silica nanofibers matrix possessed excellent catalytic properties, which may hold great promise in effective and eco-friendly waste water treatment.
University of Jos, Nigeria
Edith Adanna Onwuliri holds BPharm degree in Pharmacy and MSc degree in Applied Microbiology from the University of Jos, Plateau State, Nigeria. She has obtained her PhD in Pharmaceutics and Pharmaceutical Microbiology from the University of Nigeria, Nigeria. She is a Lecturer in the Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of Jos, Nigeria. She is interested in therapeutic investigation of local plants for their antimicrobial effects and their formulation into suitable dosage forms. She has 19 publications in various journals.
The skin is one organ which when traumatized (would/disease) can be noticed by all without asking, so people with wounds are very disturbed by their complaint in comparison with other medical conditions because the wounds tend to make the victims have a leper like complex. It is a challenge to treat wound patients because they believe as most wounds are on the surface, it should be easy to treat. This study investigated the anti-microbial and wound healing properties of acetone leaf extract and ointment formulation of Spermacoce verticillata Linn (Family Rubiaceae). Acceleration Gradient Chromatography was used to fractionate the extract into two, fractions A and B. the Cup Plate Agar Diffusion method was used to assess the antimicrobial activities of the fraction at 25, 50, 100, 200 and 400 mg/ml in comparison with 40 ug/ml gentamycin control. Fraction B had a better antimicrobial property than fraction A and this was then formulated into ointments of 0.1, 0.2, 1, 2 and 5% w/w concentrations and used for assessing wound healing on wounds inflicted on albino rats. The wounds were first infected with the Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus respectively and dressed daily with the ointment. Fraction B had better antimicrobial property than fraction A. The relationship between types of microorganisms, concentrations of ointment and wound healing time showed that wounds dressed with 2% w/w fraction B ointment healed on the 7th day for E. coli, P. aeruginosa and S. aureus infected wounds, 10 days for B. subtilis while wounds dressed with other concentrations of the formulated ointment and gentamycin ointment healed by the 10th day. Acetone leaf extract of Spermacoce verticillata possesses antimicrobial property and its fraction B promotes wound healing especially at a concentration of 2% w/v.
Southeast University, China
Naizhen Zhou has received her Master’s degree from the School of Life Science of Anhui Normal University majoring in Cytobiology. She is currently a PhD candidate of Biomedical Engineering of Southeast University, China. She has authored 3 research articles in reputed national and international journals. She is mainly working to prepare the controllable and biocompatible biomaterials as the three-dimensional cell culture scaffold, such as the poly (methyl ether vinyl-co-maleic acid)-based smart hydrogels. Her current research focus is on the influence of the three-dimensional microenvironment on the expression of cancer stem cells.
Ovarian cancer, the most lethal gynecologic malignancy in the world, is associated with an overall five-year survival rate of 30%. Although significant progress has been made in surgical resection and chemotherapy, the majority of patients suffer recurrence after surgery. Increasing evidence indicates cancer stem cells (CSCs) as the non-negligible reason for relapse of ovarian cancer, is becoming a target for anti-cancer therapies. It is considered that three-dimensional (3D) matrix replicate the in vivo tumor microenvironment more closely than the standard method, which uses two-dimensional plates. Recent evidence suggests that the components and physical properties of the microenvironment may be potential factors in the regulation of CSCs. Some strategies used the chemical and biological modification of materials to mimic the stem cell niche in order to create microenvironments that control stem cell response. Poly (methyl vinyl ether-co-maleic anhydride) (PMVE-co-MA) is an FDA approved polymer with advantage of the excellent biocompatibility and non-immunogenicity. Here we report expansion of ovarian cancer stem cell through culturing ovarian cancer cell line SKOV3 in poly (ethylene glycol) (PEG) cross-linked PMVE-co-MA hydrogels with different elastic modulus. The culturing in hydrogels can remarkably promote expression of CSCs markers, which was assayed by immunofluorescence and western blot analysis. The stiffness of the hydrogel plays a crucial role in promoting expression of CSCs markers. The hydrogel with lower elastic modulus is more beneficial to enrich CSCs in vitro. The stemness characters of SKOV3 cells cultured in the hydrogels were further confirmed through doxorubicin resistance assay, thus it might provide a valuable platform for CSCs research and anti-cancer therapeutics.
The Scientific and Technological Research Council of Turkey, Turkey
Ozlem Ertekin is currently working as a Senior Research Scientist at The Scientific and Technological Research Council of Turkey (TUBITAK), Marmara Research Center, Genetic Engineering and Biotechnology Institute, Laboratory of Diagnostic Technologies, Turkey. She has worked at several projects related with environmental and food safety as either Research Scientist or Work Package Leader. She has received her PhD at Gebze Technical University, Department of Molecular Biology and Genetics.
Aflatoxins (AFs) are toxic metabolites produced by a variety of fungi from Aspergillus spp. AF analysis is conducted with a liquid extract of the food sample to be analyzed. During the extraction process, several metabolites of the sample are co-extracted with the AF. The sample clean-up step comprises affinity based purification techniques, particularly IACs in order to concentrate AFs and purify them from complex extract matrix. In an active mycotoxin analysis laboratory, hundreds of IACs are used for sample analysis every day and IACs comprise the major cost of AF analysis. Reducing the production costs for IAC development will significantly affect AF analytical costs and increase the availability of the tests, especially in developing countries. Immunoaffinity chromatography utilizes the specificity and reversibility of antibody-antigen interactions where antibodies are immobilized to a solid support in order to create a stationary phase for chromatographic separation. So there are two main components of IACs where cost reduction studies can be focused: Antibody costs and the cost of solid support. In this study, we will present our study for the reduction of antibody cost up to 95% by changing the purification approach where we used semi-purified antibodies for IAC production. This approach not only significantly reduced costs related with consumables and personnel; it also prevented antibody losses during purification process and related activity decrease. We will evaluate the reduced antibody costs in relation with the resin costs and discuss the methods of cost reduction for chromatographic resins that comprise the solid support.