Biography
Biography: Anna V Hine
Abstract
ProxiMAX randomization is the technology that lies behind Isogenica’s Colibra™ off ering. It is a defi ned saturation mutagenesis
process that delivers precision control of both identity and relative ratio of amino acids at specifi ed locations within a protein/
antibody library. Th us unwanted amino acids such as cysteine and methionine can be eliminated from libraries because no constraints
are imposed by the genetic code. Moreover, the process is non-degenerate, which means that encoding DNA libraries are as small as is
physically possible. ProxiMAX relies on a process of saturation cycling comprising ligation, amplifi cation and digestion for each cycle
and is the science behind the commercial Colibra™ technology. Currently focused on antibody libraries but with achieved diversities
of >99% (6 & 11 saturated codons) and the potential to generate libraries of up to 1014 components, we contest that ProxiMAX
randomization is a vital tool in engineering any protein library of the highest quality. Th is presentation will examine the development
of the ProxiMAX process and give examples of libraries created to date.